(19-24 July 2010)
last monday, we sent our plasmid extraction samples (pQE30+KAS in E.coli JM109) for sequencing.
out of KAS I and KAS II, both samples gave positive result.
only KAS III gave negative result. according to First Base, they able to sequence the forward primer of pQE30+KAS III, but unfortunately they cannot sequence for the reverse primer for pQE30+KAS III. i'm not sure what's the problem. but we will send pQE30+KAS III for sequencing again. this time we try to extract a different samples and send for sequencing.
when we have confirm the sequencing result, we will proceed with transformation of pQE30+KAS into E.coli M15 and protein expression.
we try to express pQE30+KAS using two different hosts.
pQE30+KAS in E.coli JM109, and also pQE30+KAS in E.coli M15.
that's all.
will update more...
thank you.
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