KAS I
i got a new PCR primer sequence because before this, there seems to be some problems with the primers we ordered. so, once i got a new primer, i start all over again from PCR, cloning and transformation. i manage to get the gene KAS I into pQE30 plasmid and transform it into E.coli M15 and JM109 host. after i sent the plasmid for sequencing, luckily, there's no problem anymore. the sequence are okay. so now i finished with my first part. for my second part, i will start to express my KAS I protein in both E.coli JM109 host and M15 host. further progress will be updated soon.
-HANI-
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